The Tigers combined to hit five home runs in a single game for the first time since 2010.
Missouri baseball's winless week dropped it from two of four polls.
The Commodores beat the Tigers 5-2 in the series finale in Nashville, Tennessee.
Trey Harris had already tied Tuesday's game once, and he had a chance to do it again in the bottom of the ninth inning.
This week's notebook features a pitcher who sprints to the mound when he's called on to close games.
Shane Benes, son of former St. Louis Cardinals pitcher Andy Benes, made his collegiate debut in No. 18 Missouri baseball's doubleheader against Alabama. It took only three pitches for him to smack the ball against the outfield wall in center field.
Missouri hit four home runs to split a doubleheader against Alabama.
Missouri baseball overcame two errors in the top of the first inning to beat Alabama 4-3.
The Tigers have dropped to 4-4 in midweek games on the season.
Missouri baseball has already equaled its best Southeastern Conference win totals and has nearly doubled its home run total from last year.
The Tigers have their most conference series wins in a season since joining the Southeastern Conference.
The Tigers play again at 11 a.m. Saturday.
In its first game since earning a spot in most of the major college baseball polls, No. 23 Missouri fell to Tennessee 5-2 on Thursday night in Knoxville, Tennessee.
Freshman pitcher Tanner Houck has been compared to Cy Young winner Max Scherzer since he arrived at Missouri. But Tim Jamieson, doubling as the head and pitching coach for the Tigers, does not think the comparison is accurate.
Following its series win against Florida, Missouri baseball is ranked in four of the five major college baseball polls, and freshman pitcher Tanner Houck was named SEC Pitcher of the Week.
Freshman designated hitter Alex Del Rio hit an RBI-single in the bottom of the fifth inning to jump-start Missouri's offense.
Headed into Saturday's game, the Tigers had one of the worst offenses in the Southeastern Conference, but in Saturday's game Missouri racked up 10 runs on 10 hits with three home runs.
The Tigers' hitters couldn't crack Gators pitcher Logan Shore, who slipped out of trouble early and dominated Missouri late.
The loss marked the second time this season where Missouri was on the losing side of a one-run game, the first being a loss to Texas A&M-Corpus Christi in February.
The Tigers allowed more runs than in any other game this season.